Methacholine acts as a non-selective muscarinic receptor agonist to stimulate the parasympathetic nervous system. It is used to diagnose bronchial hyperreactivity, which is the hallmark of asthma and also occurs in chronic obstructive pulmonary disease. Reference standards of Methacholine API,and its pharmacopeial, non pharmacopeial impurities, and stable isotopes are listed below.
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